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Breaking New Grounds in Fluorescence Imaging: LED-induced Fluorescence (LIF) Hyperspectral Imaging

03.04.2025

 

We are excited to share that as part of the Spectral AIge research project, HAIP Solutions has pioneered new approaches in fluorescence hyperspectral imaging, making this cutting-edge technology available beyond traditional application areas.

Multispectral fluorescence microscopy with filter technology and laser-induced fluorescence (LIF) have been established for years. However, these technologies have technical limitations that make them expensive and complex to implement. Two key challenges stand out:

 

  1. Filters in Microscopy: Current systems use filters, which only allow partial spectral resolution of the fluorescence spectrum, capturing only a few spectral support points. With the advent of hyperspectral cameras, it is now possible to capture the entire fluorescence range with high spectral resolution – breaking through previous constraints.
  2. Laser-Induced Fluorescence (LIF): The complexity and high safety requirements of laser-based technology have been a significant barrier. By transitioning to LED technology with monochromatic high-power LEDs and narrowband bandpass filters, we can now achieve better results with less effort and lower safety requirements. The removal of the coherent light bundling, necessary for lasers, is a game-changer in simplifying the technology and reducing necessary safety regulations.

 

The result: By combining high-power monochromatic LEDs (up to 20 watts optical power) with hyperspectral pushbroom imagers, it is now possible to create stable LIF hyperspectral systems with reduced technical complexity.

Our research partners in this project SKZ – Das Kunststoff-Zentrum and Fraunhofer IFF will now conduct further investigations with our innovative LIF-HSI setup.

 

At HAIP Solutions, we’re excited to drive forward innovations that will unlock new possibilities for fluorescence imaging in diverse fields. Stay tuned for more advancements on the horizon! 

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